Immunohistochemical staining of formalin-fixed, paraffin-embedded cell lines and cholangiocarcinoma tissue sections with anti-major histocompatibility complex class I chain-related gene A (MICA) monoclonal antibodies. Immunoperoxidase staining was performed on formalin-fixed, paraffin-embedded Me1386 cells, HeLa cells and cholangiocarcinoma tissue sections. Left (A, C, F and I), middle (B, D, G, J and L) and right column (E, H, K and M) is Me1386 cells, HeLa cells and cholangiocarcinoma tissue sections, respectively. A and B; C, D and E; F, G and H; I, J and K and L and M were stained with WW2G8, WW6B7, WW9B8, WJ1 and MK2-23, respectively. Magnification, ×20.