Table 2.
Validation by quantitative RT-PCR
| Gene designation | Groups compared | Fold change (RT-PCR) | Fold change (Microarray) | P value* | |
|---|---|---|---|---|---|
| COL9A1 | Collagen, type IX, alpha 1 | CS > chordoma | 407.5 | 118 | 0.03 |
| COL9A1 | Collagen, type IX, alpha 1 | CS > STS | 1549.6 | 93.2 | 0.03 |
| HMW-MAA | CS > STS | 3.87 | 3.87 | 0.03 | |
| HMW-MAA | Chordoma > STS | 7.7 | 9.5 | 0.009 | |
| HMW-MAA | Chordoma = CS | 2.0 | NA** | 0.14 | |
| CD24 | CD24 antigen | Chordoma > CS | 36.3 | 27.3 | 0.01 |
| CD24 | CD24 antigen | Chordoma > STS | 50.6 | 26.5 | 0.009 |
| T | T, Brachyury | Chordoma > CS | 1072.9 | 228.1 | 0.03 |
| T | T, Brachyury | Chordoma > STS | 17157.3 | 79.7 | 0.03 |
* P values are based on RT-PCR data for the respective groups compared; ** NA HMW-MAA gene was not differentially expressed in chordoma and chondrosarcoma samples by microarray analysis