| Z-buffer (weights for 1 L) | |
|---|---|
| Na2HPO4-7H20 | 0.06 M (16.1 g) |
| NaH2PO4-H20 | 0.04 M (5.5 g) |
| KCl | 0.01 M (0.75 g) |
| MgSO4-4H20 | 0.001 M (0.25 g – use MgSO4-7H20) |
| β-mercaptoethanol | 0.05 M (3.5 mL) |
| The pH of this solution should be 7.0. Raise pH by adding NaOH and lower it by adding HCl. Store at room temperature for up to 1 year. | |