Table 1.
Synthetic oligosaccharide-polyacrylamides in form of sialoglycopolymers (conjugates with soluble polyacrylamide) used to test the receptor specificity of H1N1 viruses
Structure of oligosaccharide | Abbreviation |
---|---|
Neu5Acα2–3Galβ1–4Glcβ | 3′SL |
Neu5Acα2–3Galβ1–4GlcNAcβ | 3′SLN |
Neu5Acα2–6Galβ1–4Glcβ | 6′SL |
Neu5Acα2–6Galβ1–4GlcNAcβ | 6′SLN |
Neu5Gcα2-6Galβ1-4GlcNAcβ | 6'SLN(Gc) |
(Neu5Acα2-6Galβ1-4GlcNAcβ1-2Man)2-3,6-Manβ1-4-GlcNAcβ1-4GlcNAcβ | biantennary 6’SLN |
Neu5Acα2-6Galβ | Neu5Ac6Gal |
Neu5Acα2-6GalNAcα | SiaTn |
Neu5Gcα2-6GalNAcα | Neu5Gc-Tn |
Neu5Acα2-6Galβ1-4-(6-Su)GlcNAcβ | 6-Su-6’SLN |
Galβ1-3 | 6-SiaTF |
GalNAcα | |
Neu5Acα2-6 | |
Neu5Acα2-3 | 3,6-SiaTn |
GalNAcα | |
Neu5Acα2-6 | |
(9-NAc-Neu5Acα2-6)Galβ1-4GlcNAcβ | 6’-(Neu5,9-NAc2)LacNAc |
Neu5Acα-OCH2C6H4 | Neu5AcBn |
Sulfated trisaccharide 6-Su-6’SLN was synthesized as described previously (Pazynina et al., 2008). Biotinylated sialoglycopolymers (~30 kDa) with different saccharide residues were synthesized as previously described (Bovin et al., 1993) and were used to characterize the receptor specificity of H1N1 influenza A viruses.