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. Author manuscript; available in PMC: 2013 Oct 25.
Published in final edited form as: Virology. 2012 Jul 17;432(2):389–393. doi: 10.1016/j.virol.2012.06.018

Table 1.

Synthetic oligosaccharide-polyacrylamides in form of sialoglycopolymers (conjugates with soluble polyacrylamide) used to test the receptor specificity of H1N1 viruses

Structure of oligosaccharide Abbreviation
Neu5Acα2–3Galβ1–4Glcβ 3′SL
Neu5Acα2–3Galβ1–4GlcNAcβ 3′SLN
Neu5Acα2–6Galβ1–4Glcβ 6′SL
Neu5Acα2–6Galβ1–4GlcNAcβ 6′SLN
Neu5Gcα2-6Galβ1-4GlcNAcβ 6'SLN(Gc)
(Neu5Acα2-6Galβ1-4GlcNAcβ1-2Man)2-3,6-Manβ1-4-GlcNAcβ1-4GlcNAcβ biantennary 6’SLN
Neu5Acα2-6Galβ Neu5Ac6Gal
Neu5Acα2-6GalNAcα SiaTn
Neu5Gcα2-6GalNAcα Neu5Gc-Tn
Neu5Acα2-6Galβ1-4-(6-Su)GlcNAcβ 6-Su-6’SLN
   Galβ1-3 6-SiaTF
      GalNAcα
Neu5Acα2-6
Neu5Acα2-3 3,6-SiaTn
      GalNAcα
Neu5Acα2-6
(9-NAc-Neu5Acα2-6)Galβ1-4GlcNAcβ 6’-(Neu5,9-NAc2)LacNAc
Neu5Acα-OCH2C6H4 Neu5AcBn

Sulfated trisaccharide 6-Su-6’SLN was synthesized as described previously (Pazynina et al., 2008). Biotinylated sialoglycopolymers (~30 kDa) with different saccharide residues were synthesized as previously described (Bovin et al., 1993) and were used to characterize the receptor specificity of H1N1 influenza A viruses.