. Author manuscript; available in PMC: 2012 Aug 23.

Published in final edited form as: J Biomol Screen. 2010 Sep 20;15(9):1099–1106. doi: 10.1177/1087057110382778

Table 1.

Association and Dissociation Rate Constants of bt-TAR-32 and bt-TG6 Binding to TDP-43

Oligonucleotide	k_on (μmol⁻¹ min⁻¹)	k_off (10⁻³ min⁻¹)	K_D (nM)^a	K_D (nM)^b
TAR-32	2.4 ± 0.93	0.88 ± 0.19	0.40 (0.090–1.8)	0.75 (0.29–1.9)
TG6	4.5 ± 1.3	4.5 ± 0.49	1.1 (0.33–3.7)	0.63 (0.54–0.74)

Association and dissociation rates were determined from linear regression fit of k_obs as a function of ligand concentration where the slope is equal to k_on and the Y-intercept is equal to k_off. Rate constants expressed as the arithmetic mean ± SEM, whereas the K_D values are expressed as the geometric mean and 95% confidence intervals of 3 determinations.

Determined by the quotient of k_off/k_on, as described in the Methods section.

Determined by saturation binding, as described in the Methods section.