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. Author manuscript; available in PMC: 2012 Aug 23.
Published in final edited form as: J Biomol Screen. 2010 Sep 20;15(9):1099–1106. doi: 10.1177/1087057110382778

Table 2.

Affinities of ssDNA and ssRNA Oligonucleotides Determined by Displacement of Either bt-TAR-32 or bt-TG6 Binding to TDP-43

KD (95% CI), nM
TAR-32 TG6
ssDNA oligonucleotide
 TG12 0.0018 (0.00084–0.0038) 0.0013 (0.00047–0.0036)
 TG8 0.0058 (0.0033–0.010) 0.0064 (0.002–0.015)
 TAR-32 0.14 (0.11–0.19) 0.12 (0.074–0.21)
 TG6 0.41 (0.26–0.66) 0.29 (0.11–0.75)
 TG4 12 (5.3–26) 13 (7.8–20)
 dAC12 3300 (1500–7200) NA
 TG > 100,000 > 100,000
ssRNA oligonucleotide
 UG12 92 (47–180) 130(86–190)
 UG8 370 (160–370) 450 (270–750)
 UG6 750 (380–1500) 1300 (990–1700)
 UCUU3 14,000 (7400–27,000) 9800 (7600–13,000)
 rAC12 12,000 (4200–33,000) NA

Oligonucleotides were titrated in bt-TAR-32 and bt-TG6 binding to TDP-43 and the KD values determined as described in the Methods section. Values shown are the geometric mean and 95% confidence intervals for Between 5 and 7 determinations. NA, AC repeats hybridize with the bt-TG6 and therefore prevent the determination of affinity at TDP-43.