Table 2.
KD (95% CI), nM
|
||
---|---|---|
TAR-32 | TG6 | |
ssDNA oligonucleotide | ||
TG12 | 0.0018 (0.00084–0.0038) | 0.0013 (0.00047–0.0036) |
TG8 | 0.0058 (0.0033–0.010) | 0.0064 (0.002–0.015) |
TAR-32 | 0.14 (0.11–0.19) | 0.12 (0.074–0.21) |
TG6 | 0.41 (0.26–0.66) | 0.29 (0.11–0.75) |
TG4 | 12 (5.3–26) | 13 (7.8–20) |
dAC12 | 3300 (1500–7200) | NA |
TG | > 100,000 | > 100,000 |
ssRNA oligonucleotide | ||
UG12 | 92 (47–180) | 130(86–190) |
UG8 | 370 (160–370) | 450 (270–750) |
UG6 | 750 (380–1500) | 1300 (990–1700) |
UCUU3 | 14,000 (7400–27,000) | 9800 (7600–13,000) |
rAC12 | 12,000 (4200–33,000) | NA |
Oligonucleotides were titrated in bt-TAR-32 and bt-TG6 binding to TDP-43 and the KD values determined as described in the Methods section. Values shown are the geometric mean and 95% confidence intervals for Between 5 and 7 determinations. NA, AC repeats hybridize with the bt-TG6 and therefore prevent the determination of affinity at TDP-43.