. Author manuscript; available in PMC: 2012 Aug 23.

Published in final edited form as: Pharm Res. 2011 Apr 7;28(8):1905–1918. doi: 10.1007/s11095-011-0418-5

Table.2.

The enzyme kinetics parameters for UGT1A1 and 1A3 with appropriate flavonols

Enzymes	Substrate /Product(s)	V_max	K_m	K_s	V_max/K_m (CL_int)
Enzymes	Substrate /Product(s)	nmol/mg/min	µM	µM	ml/min/mg
UGT1A1	3,7DHF
	3-O-glucuronide	1.02 ± 0.54	1.57 ± 1.09	0.60 ± 0.38	0.65
	7-O-glucuronide	3.04 ± 0.42	1.04 ± 0.19	1.21 ± 0.23	2.92
	3,5,7THF
	3-O-glucuronide	0.82 ± 0.14	0.31 ± 0.10	2.64 ± 0.89	2.65
	7-O-glucuronide	4.59 ± 0.46	0.51 ± 0.08	1.22 ± 0.19	9.00
	3,7,4’THF
	3-O-glucuronide	0.68 ± 0.05	0.30 ± 0.05	5.41 ± 0.93	2.27
	7-O-glucuronide	1.08 ± 0.13	0.43 ± 0.10	3.53 ± 0.86	2.52
	3,5,7,4’QHF
	7-O-glucuronide	1.38 ± 0.14	0.13 ± 0.03	2.81 ± 0.67	10.62
UGT1A3	3,7DHF
	3-O-glucuronide	0.18 ± 0.02	8.81 ± 2.72	100.05 ± 94.80	0.02
	7-O-glucuronide	1.89 ± 0.36	7.02 ± 3.76	50.00 ± 80.17	0.27
	3,5,7THF
	3-O-glucuronide	0.17 ± 0.01	0.84 ± 0.18	17.86 ± 6.33	0.20
	7-O-glucuronide	3.44 ± 0.84	2.12 ± 1.24	9.95 ± 9.16	1.62
	3,5,7,4’QHF
	7-O-glucuronide	0.86 ± 0.35	3.30 ± 3.10	7.52 ± 9.58	0.26

Data are means ± S.E. of three determinations.