Table 3.

The enzyme kinetics parameters for UGT1A7~1A10 with appropriate flavonols

Enzymes	Substrate /Product(s)	Vmax	Km	Ks	Vmax/Km (CLint)
		nmol/mg/min	µM	µM	ml/min/mg
UGT1A7	3,7DHF
	3-O-glucuronide	2.04 ± 0.07	1.61 ± 0.14	309.17 ± 127.39	1.27
	7-O-glucuronide	0.44 ± 0.07	23.17 ± 6.96	N/A	0.02
	3,5,7THF
	3-O-glucuronide	3.35 ± 0.11	0.82 ± 0.08	55.75 ± 7.27	4.09
	7-O-glucuronide	2.46 ± 0.34	21.43 ± 6.09	N/A	0.11
	3,5,7,4’QHF
	3-O-glucuronide	0.77 ± 0.04	1.46 ± 0.32	N/A	0.53
	7-O-glucuronide	1.10 ± 0.11	11.56 ± 2.91	N/A	0.10
UGT1A8	3,7DHF
	3-O-glucuronide	0.76 ± 0.04	8.68 ± 1.21	N/A	0.09
	7-O-glucuronide	0.65 ± 0.10	27.32 ± 7.50	N/A	0.02
	3,5,7THF
	3-O-glucuronide	3.89 ± 0.47	6.44 ± 1.28	37.28 ± 10.12	0.60
	7-O-glucuronide	0.79 ± 0.57	4.88 ± 1.14	N/A	0.16
	3,5,7,4’QHF
	3-O-glucuronide	0.74 ± 0.04	2.39 ± 0.47	N/A	0.31
	7-O-glucuronide	1.01 ± 0.09	6.77 ± 1.78	N/A	0.15
UGT1A9	3,7DHF
	3-O-glucuronide	4.60 ± 0.28	0.22 ± 0.04	30.13 ± 7.11	20.91
	7-O-glucuronide	2.04 ± 0.09	1.50 ± 0.26	N/A	1.36
	3,5,7THF
	3-O-glucuronide	7.41 ± 0.89	0.68 ± 0.16	7.43 ± 2.12	10.90
	7-O-glucuronide	2.56 ± 0.10	0.54 ± 0.08	N/A	4.74
	3,7,4’THF
	3-O-glucuronide	2.62 ± 0.05	0.36 ± 0.03	N/A	7.28
	7-O-glucuronide	1.15 ± 0.08	2.67 ± 0.56	N/A	0.43
	3,5,7,4’QHF
	3-O-glucuronide	1.92 ± 0.03	0.32 ± 0.03	N/A	6.00
	7-O-glucuronide	0.87 ± 0.05	3.87 ± 0.59	N/A	0.22
UGT1A10	3,7DHF
	3-O-glucuronide	0.19 ± 0.01	6.66 ± 0.66	N/A	0.03
	7-O-glucuronide	0.26 ± 0.02	8.74 ± 1.37	N/A	0.03
	3,5,7THF
	3-O-glucuronide	2.01 ± 0.14	4.49 ± 0.61	73.48 ± 17.82	0.45
	7-O-glucuronide	0.77 ± 0.04	3.96 ± 0.58	N/A	0.19
	3,5,7,4’QHF
	3-O-glucuronide	1.46 ± 0.16	2.44 ± 0.55	30.01 ± 8.00	0.60
	7-O-glucuronide	1.10 ± 0.20	3.34 ± 1.21	51.37 ± 27.15	0.33

Data are means ± S.E. of three determinations. N/A means that parameter K_s was not available; this was for the cases in which data fitted better to Michaelis-Menten equation