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. Author manuscript; available in PMC: 2013 Feb 8.
Published in final edited form as: Structure. 2012 Feb 8;20(2):237–247. doi: 10.1016/j.str.2011.12.007

Figure 5. Conformational Variability of hRNAPII Revealed by ISAC Cluster Averages.

Figure 5

(A) Selected hRNAPII ISAC averages showing changes in the position/ appearance of the clamp domain (marked by yellow arrowheads).

(B) hRNAPII volumes obtained after CD-PCA analysis of resampled ISAC averages show variability in clamp structure.

(C) Two 3D maps obtained by competitive refinement of hRNAPII images using the volumes in (B) as initial references show alternative conformations of the hRNAPII clamp domain. Scale bars correspond to 10 nm in (A) and 5 nm in (B and C).