Figure 1.

KDM5A is amplified and over-expressed in human breast cancer. (A) The representative array-CGH image showing chromosome 12p and KDM5A amplification in one breast cancer sample. (B) The mRNA expression level of KDM5A was examined by qRT-PCR assays in breast cancer cells with KDM5A gain/amplification (Colo824, ZR-75-1, HCC1937, HCC1428 and SUM149) or without the gain/amplification (SUM102, SUM190 and HCC70). mRNA expression level in the MCF10A cells was arbitrarily set as 1. Significance was set as P<0.05 by the student’s t-test (P<0.05). (C) KDM5A protein levels were analyzed by Western blot in eight breast cancer cell lines with or without gene amplification, as well as in MCF10A control cells.