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. 2012 Jul 22;4(3):247–256.

Figure 2.

Figure 2

Reducing KDM5A expression by shRNA knockdown resulted in decreased cell proliferation and colony formation in soft agar. (A) Knockdown of KDM5A in four breast cancer cell lines with two different shRNAs was confirmed by Western blot assays. (B) shRNA-mediated knockdown of KDM5A inhibits cell growth in breast cancer cells with gene amplification. Cells (ZR-75-1, HCC1937 and SUM149 with KDM5A amplification, SUM102 without the amplification, as well as non-tumorigenic MCF10A) infected with control (Ctrl-sh) or KDM5A shRNAs (sh#4 and sh#5) were plated at equal density and selected with puromycin for 4 weeks. Surviving cells were stained with Crystal Violet (left panel) or counted (right panel). Relative growth was shown as the mean ± SD of triplicate determinations (*P<0.05 and ** P<0.01, Student’s t test). (C) Knockdown of KDM5A impeded the anchorage-independent growth of breast cancer cells. Relative colony number (right panel) was shown as the mean ± SD of triplicate determinations (*P<0.05 and ** P<0.01, Student’s t test).