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. 2012 Jul 22;4(3):302–315.

Figure 5.

Figure 5

ICV injection of HIV-1 Tat promotes gliosis in stable knock-down CD45 mice subjected to stable CNS CD45 knockdown (A) Confirmation by western blot of CD45 knock-down mice (CD45 RNAi) and three control groups (PBS, EV, and SCR) (n=3 for each group of mice). (B) Brain homogenates were prepared from half of brain tissues and probed by western blot using antibodies against Bcl-XL (neuronal survival) and Bax (apoptosis). The relative intensity of the western blot band ratio of Bcl-XL to Bax was significantly decreased in CD45 knock-down mice compared with scrambled mice (** p < 0.01) and other control groups. (C) TNF-α (left panel) and IL-1β (right panel) ELISA showed significantly increased CD45 knock-down mice compared with scrambled mice (SCR) and other control groups (PBS and EV) (** p < 0.001).