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. 2012 Mar;1(1):13–24. doi: 10.1002/mbo3.3

Figure 2.

Figure 2

Immunological detection of Lcp from S. erythraea pIJ6021:: lcp, S. lividans TK23 pIJ6021:: lcp, and TK24 pIJ6021:: lcp by Western blotting. The expression of Lcp in S. erythraea, S. lividans TK23, and TK24 was analyzed by SDS-PAGE. All protein solutions were obtained from cell-free concentrated extracellular supernatants of cells of S. erythraea, S. lividans TK23, and TK24 grown in LB medium at 30°C for four days. (a) Electropherogram of an SDS-polyacrylamide gel after separation of the proteins. Proteins in the gel were stained with Coomassie brilliant blue R250. (b) Western blot employing anti-Lcp-IgGs prepared from an SDS-polyacrylamide gel. Lane 1: S. erythraea harboring pIJ6021:: lcp, lane 2: S. lividans TK24 harboring pIJ6021:: lcp, lane 3: S. lividans TK23 harboring pIJ6021:: lcp, the controls are lane 4: S. erythraea harboring pIJ6021, lane 5: S. lividans TK24 harboring pIJ6021, and lane 6: S. lividans TK23 harboring pIJ6021. The approximately 43-kDa Lcp protein recognized by the anti-Lcp-IgGs is marked with an arrow.