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. 2012 Mar;1(1):13–24. doi: 10.1002/mbo3.3

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© 2012 The Authors. Published by Blackwell Publishing Ltd.

This is an open access article under the terms of the Creative Commons Attribution Non Commercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Effects of the experiment to demonstrate Lcp activity using the supernatant of Lcp-expressing strains on latex overlay agar plates. The concentrated supernatant (500 mL to 50 mL) of the mutants is shown on the panels. Both sides of the panels are furnished with the concentrated supernatant. Concentrated supernatant from (a) S. erythraea pIJ6021:: lcp, (b) S. lividans TK24 pIJ6021:: lcp, and (c) S. lividans TK23 pIJ6021:: lcp produces clear zones stainable with Schiff's reagent (right side). These strains obviously secreted a functional Lcp, as indicated by the formation of a halo. On the left, the negative control, harboring only pIJ6021 and producing no clear zones, is shown. After incubation for two to three days, agar plates were stained with Schiff's reagent to visualize aldehydes resulting from poly(cis -1,4-isoprene) cleavage.