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. 2012 Mar;1(1):33–45. doi: 10.1002/mbo3.6

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© 2012 The Authors. Published by Blackwell Publishing Ltd.

This is an open access article under the terms of the Creative Commons Attribution Non Commercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Subcellular localization of XatA and the XatA passenger domain. Proteins from the different fractions were separated on a 7.5% SDS-PAGE and subjected to Western analysis using antibodies against the XatA passenger domain (αRXatA): outer membrane (OM) (lane 1), OM vesicles (OMV) (lane 2), the secreted protein fraction (1 μl; lane 3), the secreted protein fraction (7 μl; lane 4). To determine the susceptibility of XatA to protease digestion, intact Xylella fastidiosa cells were treated with proteinase K and OM proteins (OMPs) were then isolated and subjected to Western analysis: OM from untreated cells (lane 5), OM from cells after proteinase K treatment (lane 6).