Figure 8.

Transcription analyses of yvyD after inducing the expression of ywaC. (A) Northern blot analysis of yvyD. Total RNAs were extracted from the IPTG-induced and uninduced RIK1066 and RIK1089 (aprE::Pspac-ywaC relA::erm ΔyjbM ywaC::cat spo0H::tet) cells and were used for the Northern blot analysis using a yvyD-specific riboprobe as described in the Section Experimental procedures. (B) DNA sequence of the yvyD promoter region as reported by Drzewiecki et al. (1998). –35 and –10 regions of the σ^H- and σ^B-dependent promoters are boxed using solid lines (σ^H) and dotted lines (σ^B), respectively. The two transcriptional start sites are shown in bold letters with closed (σ^H) and open (σ^B) arrowheads. (C) Primer extension products were generated using total RNAs isolated from IPTG-induced and uninduced RIK1066 or RIK1089 cells as described in the legend of Figure 6A. Closed and open arrowheads represent transcriptional start sites of the σ^H- and σ^B-dependent promoters, respectively.