Table 1.
Bacillus subtilis strains used in this study
| Strain | Genotype | Source or references |
|---|---|---|
| 168 | trpC2 | Laboratory stock |
| RIK900 | trpC2 relA::erm | Nanamiya et al. (2008) |
| RIK1000 | trpC2 ΔyjbM | Nanamiya et al. (2008) |
| RIK1003 | trpC2ΔyjbM ywaC::spc relA::erm | Nanamiya et al. (2008) |
| RIK1004 | trpC2 ywaC::cat | This study |
| RIK1051 | trpC aprE::Pspac-yjbM spc | This study |
| RIK1052 | trpC aprE::Pspac-ywaC spc | This study |
| RIK1054 | trpC relA::erm aprE::Pspac-ywaC spc | RIK900 → *RIK1052 |
| RIK1055 | trpC ΔyjbM aprE::Pspac-yjbM spc | RIK1051 → *RIK1000 |
| RIK1056 | trpC ΔyjbM aprE::Pspac-waC spc | RIK1052 → *RIK1000 |
| RIK1057 | trpC ΔyjbM ywaC::cat aprE::Pspac-yjbM spc | RIK1004 → *RIK1055 |
| RIK1058 | trpC ΔyjbM ywaC::cat aprE::Pspac-yjbM spc | RIK1004 → *RIK1056 |
| RIK1059 | trpC2 ΔyjbM ywaC::cat relA::erm aprE::Pspac-yjbM spc | RIK900 → *RIK1057 |
| RIK1066 | trpC2 ΔyjbM ywaC::cat relA::erm aprE::Pspac-ywaC spc | RIK900 → *RIK1058 |
| RIK1068 | trpC2 relA::erm ΔyjbM aprE::Pspac-ywaC spc | This study |
| RIK1069 | trpC2 yvyD::PrrnOkan | This study |
| RIK1070 | trpC2 ΔyjbM ywaC::cat relA::erm aprE::Pspac-ywaC spc yvyD::PrrnOkan | RIK1069 → *RIK1066 |
| RIK1088 | trpC2 spo0H::tet | This study |
| RIK1089 | trpC2 ΔyjbM ywaC::cat relA::erm aprE::Pspac-ywaC spc spo0H::tet | RIK1088 → *RIK1066 |
| RIK1096 | trpC2 ywaC::cat aprE::Pspac-yjbM spc | RIK1004 → *RIK1052 |
| RIK1098 | trpC2 relA::erm ywaC::cat aprE::Pspac-ywaC spc | This study |
| RIK1371 | trpC2 ΔyjbM ywaC::cat relA::erm aprE::Pspac-ywaC L176F spc | This study |
| RIK1375 | trpC2 ΔyjbM ywaC::cat relA::erm aprE::Pspac-ywaC D87G spc | This study |
| RIK1392 | trpC2 pULI7yvyD | This study |
| RIK1420 | trpC2 rrnO2+::kmpt1 | This study |
*
Transformation.