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. 2012 Jun;1(2):105–114. doi: 10.1002/mbo3.8

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© 2012 The Authors. Published by Blackwell Publishing Ltd.

This is an open access article under the terms of the Creative Commons Attribution Non Commercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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YOR387C induction by Zap1 is AD1 specific. (A) Zap1 alleles used in this study. (B) zap1Δ cells expressing either Zap1^WT, Zap1^AD1, Zap1^AD2, or the empty vector were grown to mid-log phase in LZM supplemented with either 1 μM (−) or 1000 μM (+) ZnCl₂. Total RNA was then isolated and YOR387C mRNA levels were measured by S1 nuclease protection assays. Expression of CMD1, which encodes calmodulin, was used as a loading control. (C) Cells as described in panel B bearing the YOR387C-lacZ reporter were grown to mid-log phase in LZM supplemented with either 1 μM (filled columns) or 1000 μM (open columns) ZnCl₂. Cells were then harvested, and β-galactosidase assays were performed. The values shown are the means of three independent cultures and the error bars represent ±1 SD.