Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Zap1 protein accumulation is not affected in most coactivator mutant strains. Coactivator deletion mutants that affected YOR387C-lacZ expression were grown to mid-log phase in LZM supplemented with 1 μM ZnCl2. Protein extracts were prepared and analyzed by immunoblotting using an antibody raised against the DNA binding domain of Zap1. Pgk1 phosphoglycerate kinase was used as a loading control.
