Figure 5.

Coactivator recruitment by Zap1 is strongly interdependent. Results from chromatin immunoprecipitation of Ada2-TAP (A), Spt3-TAP (B), Swi3-TAP (C), and Med15-TAP (D) are shown. Isogenic ada2Δ and swi3Δ mutants were used to determine the effects of disrupting one complex on the recruitment of others. The indicated strains were grown to mid-log phase in LZM supplemented with 1 μM ZnCl₂. Cells were then cross-linked with formaldehyde, harvested, and chromatin immunoprecipitation analysis was performed using IgG-Sepharose to immunoprecipitate TAP-tagged proteins. Coprecipitation of specific DNA fragments was then assessed by real-time PCR using primers flanking the ZREs of the ZRT1 promoter. The values shown are the means of three independent cultures and the error bars represent 1 SD.