Figure 6.

Coactivator recruitment by AD2 is weaker than AD1-mediated recruitment. Chromosomal ZAP1 was deleted from strains expressing Med15-TAP, Spt3-TAP, or Swi3-TAP. The resulting strains were transformed with Zap1^AD1, Zap1^AD2, or the empty vector and grown to mid-log phase in LZM supplemented with 1 μM ZnCl₂. These cells were then cross-linked with formaldehyde, harvested, and chromatin immunoprecipitation analysis was performed using IgG-Sepharose to immunoprecipitate TAP-tagged proteins. Coprecipitation of the ZRT1 promoter was then assessed by real-time PCR using primers flanking the ZRT1 ZREs. The values shown are the means of three independent cultures and the error bars represent 1 SD. The letters denote values that are significantly different from each other (P < 0.05) as determined using ANOVA. The dashed lines mark the background level of recruitment observed in the vector-only cells.