Figure 2.

Transcription of bclA, bapA and fimA is positively regulated by CepR. ß-galactosidase activities of transcriptional fusions of the bclA (A), bapA (B), and fimA (C) promoter regions to lacZ were assayed in the B. cenocepacia H111 wild type (filled symbols) and the isogenic cepR mutant H111-R (open symbols) throughout the growth curve (solid line). Strains harboring the P_bclA-lacZ and P_bapA-lacZ fusions were grown in unbuffered LB medium, whereas strains used to measure the activity of the P_fimA-lacZ fusion were grown in LB medium adjusted to pH 5 and 17 mM NaCl. Values are means ± SEM, n = 3.