Figure 3.

BapA has a strong influence on biofilm formation. (A) Biofilm formation in AB minimal media supplemented with 10 mM sodium citrate. After incubation at 30°C for 48 h, planktonic cells were removed and attached cells were stained with crystal violet as described in Methods section; defective biofilms were only observed with the cepR mutant H111-R and the bapA mutant H111-bapA. (B) BapA masks the effect of bclACB; bapA was expressed from a rhamnose-inducible promoter in strain H111-P_rha-bapA. Values were adjusted to percentage of the wild type (WT). Asterisk indicates statistical significance (t-test, P < 0.01). Error bars indicate SEM, n = 3.