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BapA is associated to the bacterial cell wall. B. cenocepacia wild-type strain H111 (A) and the bcam2141 transporter mutant (B) were engineered to express a mCherry-BapA fusion protein when rhamnose is present in the culture medium. Samples were grown in M9 minimal media with rhamnose as sole carbon source for maximal expression of the fusion protein. Fluorescence emission was analyzed for mCherry (left), DAPI (middle), and merged (right). Bars, 1 μm.
