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. 2012 Jun;1(2):225–242. doi: 10.1002/mbo3.24

Figure 6.

Figure 6

Subcellular localization of BclB in B. cenocepacia H111. Cells were grown on NB agar plates for 24 h at 37°C. Equivalent amounts of whole cell extracts from the wild type (WT), the lectin mutant (H111-bclACB), the complemented lectin mutant (H111-bclACB(bclACB)), and subfractions obtained from the supernatant of the cell suspension (Ext), the periplasm (Pp), cytoplasm (Cyt), and the membrane (Mem) were subjected to SDS-PAGE followed by immunoblotting using a BclB-specific antibody. The H111-bclACB strain served as negative control of the experiment.