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. 2012 Jun;1(2):149–160. doi: 10.1002/mbo3.19

Figure 3.

Figure 3

(A) Western blot analysis of whole-cell protein preparations from the Aeromonas caviae wild-type and maf1 mutant strain using polyclonal rabbit antipolar flagellin antibodies (1:1000). Lane 1, Sch3N (wild type); lane 2, maf1-; lane 3, maf1- + pBBR1MCS_maf1; lane 4, maf1- + pBBR1MCS. Whole-cell proteins were obtained from bacteria grown at 37°C in brain heart infusion broth (BHIB). (B) Western blot analysis of His-tagged flagellin protein preparations from A. caviae wild-type and maf1-harboring pSD201 (pBBR1MCS-5_hisflaA), and E. coli BL21(DE3) harboring pET28a_flaA probed with anti-Penta-His antibodies (panel 1) and anti-FlaA/B antibodies (panel 2). Lane 1, Sch3N + pSD201, lane 2, maf1- + pSD201; lane 3, BL21(DE3) + pET28a_flaA. (C) Analysis of lipopolysaccharide (LPS) isolated from A. caviae Sch3N (WT), JPS01 (maf1-), and SMT145 (lst-). LPS was extracted from bacteria grown at 37°C in BHIB, analyzed by SDS-PAGE (12%), and silver stained.