Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype and use or description | Source or reference |
|---|---|---|
| E. coli strains | ||
| DH5α | F− Phi80dlacZ ΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK-mK+) phoA supE44 lambda- thi-1; used for general cloning | Invitrogen Life Technologies™ UK |
| BL21(DE3) | F−ompT hsdSB(rB− mB−) gal dcm (DE3); used to express recombinant proteins in E. coli | Laboratory stock |
| S17–1λpir | hsdR pro recA, RP4–2 in chromosome, Km::Tn7 (Tc::Mu) λpir, Tpr Smr | De Lorenzo et al. (1990) |
| CC118 λpir | Δ(ara leu)7697 araD139 ΔlacX74 galE galK phoA20 thi-1 rspE rpoB(Rfr) argE(Am) recA1 λpir+ | Herrero et al. (1990) |
| Aeromonas strains | ||
| A. caviae Sch3N | Sch3, spontaneous Nalr | Gryllos et al. (2001) |
| A. hydrophila AH-3 | O:34, wild type | Nogueras et al. (2000) |
| A. caviae JPS01 | Sch3N; maf1::kmr | This work |
| A. caviae SMT145 | Sch3N; lst::kmr | Tabei et al. (2009) |
| Plasmids | ||
| pGEM | Cloning vector, Ampr | Promega, USA |
| pET28a | Expression vector with hexa-histidine tag, Kmr | Novagen, Merck International |
| pUC4KIXX | Source of Tn5-derived nptII gene, Kmr | Pharmacia, GE Healthcare Life Sciences, USA |
| pKNG101 | oriR6K mobRK2 strAB sacBR, 6.8 kb, Smr | Kaniga et al. (1991) |
| pBBR1MCS | Broad-host-range vector, IncP, -W, -Q, ColE1 and p15A compatible, contains pBluescript IIKS-lacZα-polylinker, Cmr | Kovach et al. (1994) |
| pBBR1MCS-5 | Broad-host-range vector, IncP, -W, -Q, ColE1 and p15A compatible, contains pBluescript IIKS-lacZα-polylinker, Gmr | Kovach et al. (1995) |
| pET28a_flaA | pET28 derivative used to express His-tagged fusion of FlaA in E. coli BL21(DE3), Kmr | This work |
| pSD201 | pBBR1MCS-5_hisflaA, Gmr | This work |
| pSRK_hisflaA | pSRK derivative used to express His-tagged fusion of FlaA in A. caviae, Gmr | This work |
| pBBR1MCS_maf1 | pBBR1MCS with A. caviae maf1and 170 bp upstream region, Cmr | This work |