Table 1.
Sequences of primers and probes for real-time PCR analysis
| Gene | Forward Primer | Reverse Primer | Probe | Accession Number |
|---|---|---|---|---|
| ARNT2 | GGTCCACTGCACAGGCTACA | CGGCATCTTCTTCAGGTATAGTCA | CAAGGCTTGGCCGCCAGCA | NM_001009452 |
| IL12B | CATCAGGGACATCATCAAACCA | CCACCTGCCGAGAATTCTTTAG | Sybr | EU340264 |
| IL18 | AATCAACCTGTCTTTGAGGATATGC | CAGACCTCTAGTGAGGCTGTCCTTATA | Sybr | NM_001009438 |
| NPY | CGGAGGACTTGGCCAGATAC | TGCCTGGTGATGAGATTGATG | ACTCAGCGCTGCGAC | NM_001009263 |
| AGRP | GAGGTGCTAGATCCGGAAGGA | TGGTGTCCCAGACAGGATTCA | CCCACGTCGCTGCGTAAGGCTG | AY310396 |
| HIF1A | GCCACAACGTCACCATATAGTGA | TCTGTCTGTTCTATGACTCCTTTTCC | AAGTCGGACAGCCTCACCCAACAGAG | AY485676 |
| TGFB1 | CAGTAAGGATAACACGCTTCAAGTG | CCGGTTCATGCCGTGAAT | ACATCAACGGGTTCAGTTCCGGCC | NM_001009400 |
| TNF | CCCTTCCACCCCCTTGTT | ATGTTGACCTTGGTCTGGTAGGA | CCACACCATCAGCCGCATTGCA | NM_001024860 |
| COPA | GAAAAACCCCACAGATGCCTAT | CCGATAAGATGCAGCACAGATG | Sybr | NM_001105645 |
| COPB1 | GGTCTGTCATGCTAATCCATCAGA | AGCAGGGCTAGATGACTGTAGTAAGTT | Sybr | NM_001078007 |
| CCL3 | GGTGTCATCTTCCAGACCAAAAA | TCCTGGACCCAGTCCTCAGT | Sybr | NM_174511 |