Fig. 1.

Expression of p53 in K562 cells, a p53⁻ cell line. P53-1 and p53-2, 2 stable wild-type p53⁺ K562 cell lines, and MT, an empty vector-transduced K562 cell line, were generated. K562 cells were stimulated with 10 ng/ml PMA to induce terminal megakaryocytic differentiation. Transcript level expression of p53 (A) and p53 targets CDKN1A (p21) (B), BAX (C), and ACTA2 (D) was measured by Q-RT-PCR on days 1, 3, 5, and 7 following PMA stimulation. Error bars: SE, n = 2. Western blot confirming p53 protein expression in p53-1 (E) K562 cells relative to MT cells on days 1, 3, 5, and 7 following PMA stimulation. Shown is 1 of 2 biological replicates.