Table 1. Primer sequences used in real-time PCR for quantitative gene expression analysis.
Genes | Accession # | Primer 5′ – 3′ | PCR Product (bp) |
GAPDH | BC 020308 | F: 5′-tcc ctg agc tga acg gga ag-3′ | 217 |
R: 5′-gga gga gtg ggt gtc gct gt-3′ | |||
Type III collagen | NM 000090 | F: 5′-gtt gac cct aac caa gga tgc a-3′ | 203 |
R: 5′-gga agt tca gga ttg ccg tag-3′ | |||
Keratin 10 | NM 000421 | F: 5′-gag caa gga act gac tac ag-3′ | 249 |
R: 5′-ctc ggt ttc agc tcg aat ct-3′ | |||
Keratin 14 | BC 002690 | F: 5′-aga acc gca agg atg ccg ag-3′ | 150 |
R: 5′-act gca gct caa tct cca gg-3′ |
Data from Genbank database sequence.