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. 2012 Aug 23;7(8):e43664. doi: 10.1371/journal.pone.0043664

Table 2. Overview of all results including the corresponding significances.

SW-684 SW-982 SW-1353
Method ALDH1high ALDH1low ALDH1high ALDH1low ALDH1high ALDH1low
% ALDH1high cells 1.77±0.9 2.23±1.0 2.69±1.3
Aldefluor® Assay
% ALDH1high cells 41.7±18.6 52.5±8.7 31.7±11.1
Repopulation Assay p = 0.0039 p = 4.4E-7 p = 0.0025
expression (%) 67.17% 32.83% 55.34% 44.66% 72.57% 27.43%
ALDH1 western blot
Ki-67 positivity 0.85±0.17 0.38±0.05 0.59±0.31 0.35±0,27 0.98±0.01 0.18±0.15
Quantitation IHC
CFE % 4.95±2.59 5.16±2.25 19.0±3.42 15.8±7.66 10.5±2.74 6.5±2.41
colony formation assay p = 0.0047
c-Myc 1.72±1.44 1.33±1.04 1.52±0.69 0.99±0.40 2.33±1.77 0.82±0.33
relative gene expression
β-catenin 0.98±0.82 0.87±0.87 0.41±0.06 0.81±0.18 1.16±0.13 0.95±0.37
relative gene expression
SOX-2 25.2±19.4 9.66±2.77 1.96±1.29 1.02±0.59 4.37±3.83 39.82±28.61
relative gene expression
ABCG2/BCRP1 2.42±1.56 1.05±0.15 2.28±0.78 1.15±0.29 4.38±0.73 1.76±0.23
relative gene expression p = 0.0143 p = 0.0047 p = 0.0227
ABCA2 1.04±0.34 0.94±0.32 3.42±1.37 1.32±0.25 1.88±0.41 0.62±0.17
relative gene expression
ABCB1/MDR1 1.66±0.61 1.14±0.28 2.25±1.03 1.08±0.27 11.15±2.55 1.52±0.22
relative gene expression p = 0.0302
doxorubicin IC50 1.5 µM 1.4 µM 1.8 µM 0.9 µM 1.1 µM 0.7 µM
drug sensitivity
epirubicin IC50 2.2 µM 2.0 µM 1.3 µM 0.5 µM 1.5 µM 0.7 µM
drug sensitivity
cisplatin IC50 25.7 µM 24.9 µM 23.7 µM 18.8 µM 30.7 µM 26.2 µM
drug sensitivity