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. 2012 Aug 23;8(8):e1002882. doi: 10.1371/journal.ppat.1002882

Figure 6. The detection of polysaccharides on the hyphal cell walls of C. miyabeanus.

Figure 6

α- and β-1,3-glucan were detected using fluorophore-labeled antibodies, and chitin was detected using a fluorophore-labeled lectin. (A) The detection of polysaccharides at the accessible surface of the cell wall of mycelia developed on glass coverslips. Representative images from more than 100 fungal cells from two independent experiments are presented. (B) The detection of polysaccharides at the accessible surface of the cell wall of infectious hyphae developed on rice sheath cells. Layered images of α- and β-1,3-glucan are presented as ‘α-1,3-glucan+β-1,3-glucan’. Representative images of 50 fungal cells from 10 independent experiments are presented. (C) The detection of polysaccharides in the cell wall of infectious hyphae treated with α-1,3-glucanase. The representative images of 50 fungal cells from 10 independent experiments are presented. BF, bright-field optics; AP, appressorium; IH, infectious hyphae; CO, conidia. Scale bar = 20 µm.