Figure 6. The kinase inactive mutant, CRN8^R469A;D470A, destabilizes the CRN8 protein.

(A) Overview of the different combinations of Agrobacterium-mediated co-expressed proteins in N. benthamiana leaves. (B) Macroscopic cell death 5 days post infiltration in N. benthamiana leaves; strongest cell death classified as ++ and suppressed cell death as +/−. (C) Western blot of FLAG:CRN8 protein inputs probed with FLAG antibody. (D) Coomassie stain indicating equal loading of protein on Western blot depicted in figure 6C. (E) Western blot of GFP, GFP:CRN8, and GFP:CRN8^R469A;D470A signals from crude protein extracts probed with GFP antibody. (F) Coomassie stain indicating equal loading of protein on Western blot depicted in figure 6E.