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. 2012 Aug 23;8(8):e1002875. doi: 10.1371/journal.ppat.1002875

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© 2012 van Damme et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Overview of the different combinations of FLAG:CRN8, GFP:CRN8, FLAG:CRN8^R469A;D470A, and GFP (indicated by +) proteins, co-expressed in N. benthamiana leaves. (B) Western blot of GFP:CRN8 and GFP protein from GFP immuno-purified protein samples probed with GFP antibody. (C) Western blot of FLAG:CRN8 and FLAG:CRN8^R469A;D470A inputs for GFP co-immunopurification experiment probed with FLAG antibody. (D) Co-immunoprecipitation of FLAG:CRN8 and FLAG:CRN8 ^R469A;D470A in GFP immuno-purified protein samples on a Western blot probed with FLAG antibody. (E) Coomassie stain indicating equal loading of protein on Western blot in figure 7D.