Figure 1. Fertility, litter size and survival of fetuses/pups in Gcg^gfp/gfp mice.

(A) Pregnancy rate in the control (open squares: Gcg^+/+ and Gcg^gfp/+) and Gcg^gfp/gfp (closed squares) mice. Numbers in brackets indicate mating. The rate was not affected by genotype of the male (data not shown). ***p<0.01 by the chi-square test. (B) Litter size in the control and Gcg^gfp/gfp (closed squares) mice at postnatal day 0 and 21. Numbers in brackets indicate litters analyzed. Values are expressed as means±SEM. ***p<0.01 by one-way ANOVA and t-test. (C–E) Survival of fetuses. Female mice were sacrificed at gestational day 9.5 and 15.5. Numbers in brackets indicate number of pregnant females analyzed. Numbers of viable fetuses (C), resorbed fetuses (D) and % resorption (E) are shown. Values are expressed as means±SEM. ***p<0.01 by one-way ANOVA and t-test. (F) Rate of losing litters within 36 hours after delivery. Values are expressed as means±SEM. ***p<0.01 by one-way ANOVA and t-test.