Figure 3. XB130 binds to p85α subunit of PI3K and controls Akt activity in cancer cells.

(A) Schematic representation of protein structures of XB130 and p85α subunit of PI3K. Interactions of XB130 with p85α were detected by co-immunoprecipitation in WRO and A549 cells. (B) XB130 siRNA effectively reduced XB130 protein levels and Akt phosphorylation in WRO and A549 cells. siRNA transfected cells were harvested at 48 h after transfection. n = 5. *p<0.05 (compared with control siRNA).