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. 2012 Aug 23;8(8):e1002876. doi: 10.1371/journal.ppat.1002876

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© 2012 Krumm et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Biotinylated IL18 was captured on a BIAcore streptavidin-coated CM5 chip, and its binding with IL18BP was monitored with a BIAcore 3000 sensor. All the YLDV-IL18BP proteins were expressed in E. coli as SUMO fusion and purified to near homogeneity. The IL18BP mutants were derived from the monomeric form of the protein with the HVEC mutation. The injection of IL18BP started at ∼150 s and stopped at 900 s. The colored lines are the responses obtained with different IL18BP mutants and normalized to a maximum of 100 resonance units (RU) (except for Y56A) for ease of comparison.