Table 3. Kinetics and affinity constants of the binding of IL18 mutants with immobilized IL18BPs.
| YLDV-IL18BP | ECTV-IL18BP | |||||
| IL18 mutantsa | KOn,105/Ms | Koff,10−4/s | KD, nM | KOn,105/Ms | Koff,10−4/s | KD, nM |
| WT | 1.2±0.1 | 0.2±0.1 | 0.2±0.1 | 1.7±0.3 | 0.4±0.1 | 0.2±0.1 |
| Y1A | 1.1±0.2 | 4.5±0.5 | 4±1 | 1.7±0.5 | 0.5±0.1 | 0.3±0.1 |
| K53A | 1.1±0.1 | 6.3±0.8 | 6±1 | NFb | ||
| P57R | NFb | 0.4±0.2 | 8±2 | 25±7 | ||
| S105R | 1.3±0.1 | 8.3±0.1 | 6.7±0.6 | 3.5±0.4 | 0.2±0.1 | 0.1±0.1 |
| P57R,S105R | NBc | 0.9±0.3 | 3.9±0.4 | 4±1 | ||
| D110A | 1.0±0.1 | 1.1±0.1 | 1.1±0.1 | 2.6±0.2 | 0.3±0.1 | 0.2±0.1 |
a
The kinetics and affinity constants were derived from 2 independent experiments similar to those shown in Figure 10. K values are means ± standard deviations.
b
NF: binding data did not fit 1∶1 binding model.
c
NB: no binding.