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. 2012 Aug 23;8(8):e1002890. doi: 10.1371/journal.ppat.1002890

Figure 2. ADCC titers elicited by SIVmac239Δnef versus single-cycle SIV.

Figure 2

Plasma samples collected on weeks 2 and 12 after a series of inoculations with single-cycle SIV were titered for ADCC against SIVmac239-infected cells (A). Target cells infected with SHIVSF162P3 served as a negative control (gray). Dashed lines indicate 50% activity. Geometric mean vaccine strain viral loads reflecting virus particles produced in vivo after inoculation with SIVmac239Δnef or single-cycle SIV are shown (B). Animals in Group A were inoculated 3 times with single-cycle SIV that was trans-complemented with the vesicular stomatitis virus glycoprotein (VSV G), whereas the animals in Group B were inoculated 6 times with single-cycle SIV that was not trans-complemented [38]. The 50% ADCC titers (C) and the AUC values for ADCC (D) elicited by SIVmac239Δnef were significantly higher than those elicited by single-cycle SIV (2-tailed Mann-Whitney U tests, P = 0.0062 to P<0.0001). Binding titers measured by ELISA against SIVmac239 gp120 were correlated with 50% ADCC titers (E), and with AUC values for ADCC (F). Binding titers against SIVmac239 gp140 were also correlated with 50% ADCC titers (G), and with AUC values for ADCC (H).