Table 1.

Photophysical Data for nucleosides 1–4

compound	solvent	λabs (nm)a	λem (nm)	ε b	Φ c	Stokes shiftd	brightness (Φε)	polarity sensitivitye
PydC	H2O	342	461	4.03	0.05	7.6	0.20	71
	Dioxane	351	439	5.00	0.10	5.7	0.50
PyC	H2O	343	463	5.04	0.04	7.6	0.20	67
	Dioxane	349	439	5.00	0.10	6.0	0.50
1	H2O	369	469	11.6	0.41	5.7	4.8	70
	Dioxane	383	449	13.6	0.50	3.9	6.8
2	H2O	371	473	12.9	0.43	5.9	5.5	73
	Dioxane	379	449	15.0	0.47	4.0	7.1
3	H2O	357	474	2.38	0.01	7.0	0.024	75
	Dioxane	367	447	3.16	0.70	4.9	2.2
4	H2O	357	478	2.42	0.01	7.1	0.024	74
	Dioxane	365	447	3.21	0.74	5.0	2.4

^aLong λ maximum.

^b10³ M^–1cm^–1

^cRelative quantum yields

^d10³ cm^–1

^ePolarity sensitivity values are expressed in cm^–1/(kcal·mol^–1) and represent the slope of the line in the plot of stokes shift versus solvent polarity (see Supporting Information for details)