Table 3.

Kinetic parameters for the metabolism of (S)- and (R)-norKet in pooled HLMs (n=2). A range of (S)- and (R)-norKet concentrations was incubated with HLMs (0.5 mg/ml) at 37°C for 30 min. Substrate concentrations versus velocity were fit using Michaelis-Menten nonlinear regression analyses, where: Vmax, pmol/min/mg protein; Km, μM; and Vmax/Km (in vitro intrinsic clearance, Clint),μL/min/mg protein.

Metabolite	(S)-norKet			(R)-norKet
	Vmax	Km	Vmax/Km	Vmax	Km	Vmax/Km
HNK
4a	196.3	76.2	2.58	366.7	96.1	3.82
4c	837.3	75.8	11.0	825.4	54.3	15.2
4d	437.5	97.0	4.5	127.2	199.8	0.64
4e	14.3	62.1	0.23
4f	213.2	39.6	5.39	488.8	111.2	4.39
DHNK
DHNK	437	112.6	3.88	469	145.2	2.23