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Transamination of 5 natural amino acids in L. lactis ILCitM(pFL3)
| Amino acida | Amt (μM)b |
|
|---|---|---|
| Asp | Ala | |
| Met | 124 ± 13 | 13 ± 10 |
| Phe | 70 ± 20 | 5 ± 3 |
| Val | 56 ± 13 | 8 ± 7 |
| Ile | 50 ± 6 | 6 ± 4 |
| Leu | 51 ± 9 | 7 ± 3 |
Amino acids were added at a concentration of 2 mM in the presence of 2 mM citrate and 50 μM PLP.
Amounts of Asp and Ala were measured after 3 h of citrate-driven transamination; the amounts of Asp and Ala produced from citrate in the absence of added amino acids were subtracted.
