Table 1.
Two-way PERMANOVA of binary DGGE profiles and 16S rRNA gene pyrosequencing samples, based on SILVA database-derived OTUs (>97% identity) and a Jaccard distance matrixa
| Study method and variable | df | SS | MS | F value | P(MC) |
|---|---|---|---|---|---|
| DGGE | |||||
| Coral | 1 | 13044 | 13044 | 4.5742 | <0.01 |
| Sites | 3 | 22,929 | 7,642.9 | 2.6801 | <0.01 |
| Coral × site | 3 | 21,967 | 7,322.3 | 2.5677 | <0.01 |
| Residual | 44 | 1.25E+05 | 2,851.7 | ||
| Total | 51 | 1.87E+05 | |||
| Pyrosequencing | |||||
| Coral | 1 | 6,750.20 | 6,750.20 | 3.86 | 0.02 |
| Site | 3 | 6,333.40 | 2,111.10 | 1.21 | 0.34 |
| Coral × site | 3 | 5,878.10 | 1,959.40 | 1.12 | 0.44 |
| Residual | 3 | 5,244.80 | 1,748.30 | ||
| Total | 10 | 25,949.00 |
a
The analysis was performed at the order level. See Table S3 in the supplemental material for pairwise comparison results. SS, sum of squares; MS, mean square. P values were based on the Monte Carlo statistic, P(MC), and on Jaccard distances. Values in bold are statistically significant.