Fig 4.

Procrustes rotation analysis of microbiota composition against serum metabolite profiles (A) and microbiota composition against gene expression (B) in conventional rats fed BD or RS for 28 days. The circles represent ordination projections: microbiota (black), serum metabolites (green), and gene expression (yellow). The gene expression and metabolite data used for analyses were those that had the top 1% variation in gene expression levels and the top 15% variation in metabolite intensities across all samples. Each line connects the microbiota projection to the metabolite projection (A) or gene expression projection (B) for each sample, with decreasing length indicating increasing similarity between projections. The overall correlations between rotations were 0.59 (A) and 0.74 (B). The line colors indicate the diet: BD (blue) and RS (red).