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Comparison of ELISA (A) and ECL assay (B) for the detection of various B. anthracis strains and B. thuringiensis strain 9727. Spores were diluted in EIA buffer and assayed using simultaneous procedures with a 2-h incubation time. The same pair of antibodies was used, with A1 as the capture antibody and R93 as the tracer antibody. The nonspecific binding of each MAb was subtracted. OD, optical density.
