Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype/comments | Source |
|---|---|---|
| Plasmids | ||
| pKD46 | λ-RED (gam bet exo) araC rep101(Ts) Ampr | 10 |
| pIJ773 | λ-RED (gam bet exo) araC rep101(Ts) Ampr | 23 |
| pUZ8002 | tra neo RP4, Kmr | 57 |
| pUC-Hy | Source of hyg gene | 38 |
| RF00131J24 | Fosmid clone carrying sigG, Cmr | 71 |
| RFMD5 | RF00131J24 with cat replaced with hyg, Hmr | This study |
| RFMD6 | RFMD5 with sigG replaced with Apra cassette, Hmr Aprar | This study |
| Strains | ||
| E. coli BW25113 | K-12 derivative: ΔaraBAD, ΔrhaBAD Ampr | 23 |
| E. coli DH10B | Host for pUZ8002 and RFMD4, RFMD6, RFMD8, RFMD10, Kmr | 23 |
| RHA1 | Rhodococcus jostii RHA1, wild type | 65 |
| RHA1_013 | Derivative of RHA1; ΔsigG; Aprr | This study |