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. 2012 Aug 15;26(16):1837–1850. doi: 10.1101/gad.195123.112

Figure 3.

Figure 3.

Expression analysis of 250pMEA∷GUS in mea-1 and met1-3 mutants. (A) Percentage of seeds expressing 250pMEA∷GUS from before fertilization (BF) until 4 DAP. Reciprocal crosses were made between plants homozygous for 250pMEA∷GUS and mea-1/mea-1 (mea) plants. Error bars indicate SEM of two biological replicates. (n) Total number of seeds. (B) Maternal 250pMEA∷GUS expression was detected throughout seed development (top row), and weak paternal 250pMEA∷GUS expression was first detected after 3 DAP (bottom row). (C) Percentage of seeds expressing 250pMEA∷GUS from before fertilization (BF) until 4 DAP. Reciprocal crosses were made between met1-3/MET plants hemizygous for 250pMEA∷GUS (met; 250pMEA∷GUS) and Ler wild-type plants (WT). Error bars indicate SEM of two biological replicates. (n) Total number of seeds. (D) Maternal 250pMEA∷GUS expression was detected throughout seed development (top row), and no paternal 250pMEA∷GUS expression was detected (bottom row).