Skip to main content
. 2012 Aug 15;26(16):1864–1873. doi: 10.1101/gad.195412.112

Figure 3.

Figure 3.

Polarity of tandem UAA codons in the chiP gene. The sequence UAAUAA was inserted in place of tandem sense codons at different positions of the chiP gene in the chromosome of a strain carrying chiQ-lacZ. (A) Effect of rho mutation on the polarity gradient. Loss of lacZ stimulation by rho G63D in the interval between UAA-63 and UAA-108 (from 5.6-fold to 2.3-fold) suggests the presence of a polarity site within this interval. Strains used were MA10802 (UAA-10), MA10960 (UAA-46), MA10961 (UAA-63), MA11046 (UAA-78), MA11047 (UAA-108), and MA10445 (UAA-256). (B) Nucleotide sequence of the 5′ portion of the chiPQ mRNA. The ChiX pairing sequence and chiP-initiating AUG are boxed in yellow and orange, respectively. Gray boxes denote the positions of the double UAA codons. An octameric rut site element and the sequences found at the 3′ ends of in vitro terminated transcripts (see the text) are boxed in green and red, respectively.