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. 2012 Aug 15;26(16):1864–1873. doi: 10.1101/gad.195412.112

Figure 5.

Figure 5.

Effect of Rho and NusG on in vitro transcription of DNA templates containing the upstream section (ChiP1 template) or the entirety (ChiP2 template) of the chiP gene fused to the T7A1 promoter. Runoff (RO) transcripts are 560 and 1575 nt long for transcriptions with ChiP1 (A) and ChiP2 (B), respectively. Longer transcripts presumably result from RNA polymerase continuing transcription after reaching the end of a DNA template and “switching” to another template molecule (Nudler et al. 1996; Rabhi et al. 2011). The concentrations of Rho hexamers and Bcm were 70 nM and 150 μM, respectively. The concentration of NusG was 70 nM (+ lanes) or 140 nM (++ lanes).