Fig. (2).

In situ hybridization analysis of DNA probes prepared from BAC clones. The BAC clones RP11-294C12 and RP11-242E13 hybridized to metaphase spreads prepared from short term cultures of human lymphocytes showed specific hybridization to the target regions on the X (arrowhead) and Y (arrow) chromosome, respectively. A) Schematic representation of the FISH target regions on the X and Y chromosome. B) Hybridization of both probes to metaphase chromosomes. C) Hybridization signals in diploid interphase cell nuclei. (Bars = 10 µm).